| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Cadence C (9)
|
GUSHAC |
35.64 |
35.86 |
0.22 |
| 2 |
Anderson, Laine M (10)
|
GUAQUA |
36.37 |
36.78 |
0.41 |
| 3 |
Ceraudo, Savannah G (10)
|
GUSHAC |
38.10 |
38.41 |
0.31 |
| 4 |
Rayburn, Myah A (10)
|
GUAQUA |
37.65 |
39.14 |
1.49 |
| 5 |
Ceraudo, Olivia M (10)
|
GUSHAC |
|
40.93 |
- |
| 6 |
Yamokoski, Julia E (10)
|
GUAQUA |
48.33 |
42.72 |
-5.61 |
| 7 |
Morgan, Jayde K (9)
|
GUSHAC |
44.91 |
44.93 |
0.02 |
| 8 |
Stapleton, Tallis M (8)
|
GUSHAC |
43.09 |
45.43 |
2.34 |
| 9 |
Sosa, Lyza A (7)
|
GUSHAC |
49.47 |
47.10 |
-2.37 |
| 10 |
Miller, Evie M (10)
|
GUSWAT |
53.56 |
47.93 |
-5.63 |
| 11 |
Cassady, Samantha M (10)
|
GUAQUA |
49.53 |
49.00 |
-0.53 |
| 12 |
Schauss, Ingrid A (8)
|
GUAQUA |
49.58 |
49.03 |
-0.55 |
| 13 |
Laurito, Carmen S (9)
|
GUAQUA |
48.94 |
50.05 |
1.11 |
| 14 |
Herbst, Grace E (10)
|
GUSHAC |
55.83 |
52.45 |
-3.38 |
| 15 |
Harris, Trinity L (10)
|
GUSSS |
55.19 |
52.89 |
-2.30 |
| 16 |
Adodo, Angelika (9)
|
GUCOOG |
50.45 |
53.00 |
2.55 |
| 17 |
Martinez, Azul (8)
|
GUHCAP |
52.59 |
55.84 |
3.25 |
| 18 |
Busby, Mia B (6)
|
GUSHAC |
|
56.12 |
- |
| 19 |
Prior, Charlotte G (9)
|
GUSSS |
|
57.34 |
- |
| 20 |
Tinson, Holly F (10)
|
GUAQUA |
42.55 |
57.78 |
15.23 |
| 21 |
Fontenot, Kalyssa M (7)
|
GUSHAC |
|
58.85 |
- |
| 22 |
Reichert, Anna E (8)
|
GUAQUA |
55.47 |
58.94 |
3.47 |
| 23 |
Rodriguez, Bridget A (10)
|
GUHCAP |
|
59.12 |
- |
| 24 |
Boettcher, Kyleigh M (10)
|
GUSWAT |
|
1:02.35 |
- |
| 25 |
Camacho-Molinero, Karen (10)
|
GUHCAP |
1:23.23 |
1:05.63 |
-17.60 |
| 26 |
Salinas, Gabriela (9)
|
GUHCAP |
|
1:06.08 |
- |
| 27 |
Willemse, Tara A (9)
|
GUCOOG |
|
1:06.46 |
- |
| 28 |
Sanders, Ava E (9)
|
GUCOOG |
1:15.23 |
1:06.74 |
-8.49 |
| 29 |
Halvorsen, Karley R (9)
|
GUCOOG |
|
1:07.62 |
- |
| 30 |
Zamarripa, Trinity (9)
|
GUHCAP |
1:08.19 |
1:19.32 |
11.13 |
| 31 |
Liu, Tegan W (7)
|
GUAQUA |
|
1:21.76 |
- |
|
Menifee, Mikinzi G (10)
|
GUHCAP |
|
DQ |
- |
|
Coward, Kayin S (10)
|
GUHCAP |
|
NS |
- |